By Stephen Welle (auth.), Andrei Y. Yakovlev, Lev Klebanov, Daniel Gaile (eds.)
Microarrays for simultaneous dimension of redundancy of RNA species are utilized in primary biology in addition to in scientific examine. Statistically,a microarray might be regarded as an commentary of very excessive dimensionality equivalent to the variety of expression degrees measured on it. In Statistical tools for Microarray facts research: tools and Protocols, specialist researchers within the box element many tools and strategies used to review microarrays, guiding the reader from microarray know-how to statistical difficulties of particular multivariate information research. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, the chapters contain the type of precise description and implementation recommendation that's the most important for buying optimum ends up in the laboratory.
Thorough and intuitive, Statistical equipment for Microarray facts research: Methods and Protocols aids scientists in carrying on with to review microarrays and the most up-tp-date statistical methods.
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Additional info for Statistical Methods for Microarray Data Analysis: Methods and Protocols
2. Frustrations Also, in the decade since the advent of microarray technology a great deal of frustration has accumulated among biologists who have dedicated their efforts in following up false research directions. Many articles have been discredited; scientists have difficulties in finding studies that point to something concrete and in validating the results and several articles show this frustration (60–62). Several studies (63–65) have found that the list of differentially expressed genes has had very low overlap between different platforms.
The first one is microarray reliability. The MACQ project currently addresses the evaluation of the repeatability within a single site, between sites reproducibility and comparability between platforms. Although some researchers believe that experiments addressed in the MAQC project are conducted in conditions too “ideal” and that hardly reflect the real situation of many experimental laboratories (74) and sample sizes are not large enough (75), in general the results are satisfactory (76). Moreover, this technology is constantly evolving and improving, so it is expected to provide better and lower budget results.
Nat Biotechnol 24(9):1151–1161 68. Bosotti R et al (2007) Cross platform microarray analysis for robust identification of differentially expressed genes. BMC Bioinform 8(Suppl 1):S5 69. Wang Y et al (2006) Large scale real-time PCR validation on gene expression measurements from two commercial long-oligonucleotide microarrays. BMC Genomics 7:59 70. Kuo WP et al (2006) A sequence-oriented comparison of gene expression measurements across different hybridization-based technologies. Nat Biotechnol 24(7):832 71.