By Brillouin L.
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Extra info for Is It Possible to Test by a Direct Experiment the Hypothesis of the Spinning Electron
Constructs have been generated where the repeats have been moved downstream, upstream, inverted, and varied in number (37). They have also been placed adjacent to other toxoplasma "promoters" or put upstream of a reporter with no promoter. Briefly, the results show that these are best described as promoter elements because they are absolutely necessary for transcription of the SAG1 locus and their position determines the start site for transcription. However, they can function in either orientation, which is highly unusual for a promoter and, when placed upstream of a heterologous promoter (a truncated promoter of TUB1), they can result in substantially more activity (37).
Gondii are capable of crossing in a productive way. Although expected, such a cross had never been described before; it was possible that the parasite populations are in the process of speciating and that two random strains might not cross. All of the above refers, of course, only to the nuclear genome. In addition to this there are believed to be at least two nonnuclear genomes (17, 18) originating, respectively, from the mitochondrion and an as yet uncharacterized organelle (that may be plastid-like).
C. M. Gorman, Mol. Cell. Biol. 2, 1044 (1982). J. R. Neumann, C. A. Morency, and K. O. Russian, Biotechniques 5, 444 (1987). D. Soldati and J. C. Boothroyd, manuscript in preparation. A. L. ten Asbroek, C. A. Mol, R. Kieft, and P. Borst, Mol. Biochem. Parasitol. 59, 133 (1993). K. Kim and J. C. Boothroyd, manuscript in preparation. L. H. Kasper, Parasitol. Immunol. 9, 433 (1987). L. H. Kasper, I. A. Khan, K. H. Ely, R. Buelow, and J. C. Boothroyd, J. Immunol. 148, 9805 (1992). K. Kim, D. Soldati, and J.