Human Oocytes and Their Chromosomes: An Atlas by B.-M. Uebele-Kallhardt

By B.-M. Uebele-Kallhardt

The decade has noticeable impressive advances in human ge­ netics. as soon as the right kind chromosome variety of the human genome was once ascertained, a large choice of ailments used to be rec­ ognized as because of numerical chromosome anomalies. There the invention that spontaneous abortions are the results of chromosome mistakes, and particular band styles of chromosomes allowed identity of minute lesions. The concepts of telephone hybridization now enable particular gene assign­ ment to chromosomes or even to distinctive loci on their fingers. All this was once attainable due to the ease with which metaphase chromosomes may be got and manipulated. The a lot older means of research of meiotic chromosomes has taken a again seat during this intriguing period. Being less with no trouble accessi­ ble, spermatogonial research is far much less often lower than­ taken and is much less winning. much more tricky for research is the feminine meiotic strategy. not just is meiosis extraordinar­ ily lengthy, spanning from sooner than beginning to ovulation, the tech­ niques for its research and the endurance required for special inquiry were major hindrances. even as, the suspicion that girl meiotic research are not merely be profitable yet that it can be necessary has been with us ever because it was once well-known optimistic correlation exists among chromosomal nondisjunction and maternal age. sooner than the intricacies of chromosomal habit which are re­ sponsible for nondisjunction are understood, despite the fact that, it is vital that we understand the normalcy of the method.

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X 400 58 Fig. 31 A Fig. 31 B 59 Follicular Cell Culture Fig. 32. After removal of the oocyte, the follicle cells were maintained in a longer term tissue culture. Until day 7 the epithelial cells continue to grow as monolayers x480 Fig. 33.

Boyer, L. ). : Charles C Thomas, 1972, pp. 37-42. : Role of fetal follicular cells in meiosis of mammalian oocytes. Cytogenetics 3, 324-333 (1964). : Human Oogenesis. Cytogenetics 1,42-51 (1962). : Nucleolus-Organizers in the causation of chromosomal anomalies in man. Lancet II 1961, 123-126. : Female germ cells of man. Exp. Cell Res. 24, 106-110 (1961). : Nucleoli, the cellular sites of ribosome production. In: Handbook of Molecular Cytology, Lima de Faria, A. , 1969, pp. 620-636. : Individualisation d'un stade preleptotene de condensation chromosomique au debut de la meiose chez l'ovocyte foetal humain.

31 A and B. Four primary oocytes in culture. A. 24 h after incubation, the follicular cells of corona radiata are grown in dense layers. B. Enlargement of a portion of A. A. x 120; B. x 400 58 Fig. 31 A Fig. 31 B 59 Follicular Cell Culture Fig. 32. After removal of the oocyte, the follicle cells were maintained in a longer term tissue culture. Until day 7 the epithelial cells continue to grow as monolayers x480 Fig. 33.

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