Handbook of Genetics: Volume 5: Molecular Genetics by Neil A. Straus (auth.), Robert C. King (eds.)

By Neil A. Straus (auth.), Robert C. King (eds.)

Many glossy geneticists try and elucidate the molecular foundation of phenotype through the use of a battery of options derived from actual chemistry on subcellular parts remoted from a variety of species of organisms. quantity five of the guide of Genetics offers factors of the benefits and shortcomings of a few of those progressive tech­ niques, and the nonspecialist is alerted to key examine papers, reports, and reference works. a lot of the textual content offers with the constitution and func­ tioning of the molecules bearing genetic info which dwell within the nucleus and with the processing of this data by means of the ribosomes resid­ ing within the cytoplasm of eukaryotic cells. The mitochondria, which additionally stay within the cytoplasm of the cells of all eukaryotes, now seem to be separate little creatures. those, as Lynn Margulis mentioned in quantity 1, are the colonial posterity of migrant prokaryotes, most likely primitive micro organism that swam into the ancestral precursors of all eukaryotic cells and remained as symbionts. they've got maintained themselves and their methods ever when you consider that, replicating their very own DNA and transcribing an RNA rather diverse from that in their hosts. In an identical demeanour, the chloroplasts in all vegetation are self-replicating organelles possibly derived from the blue-green algae, with their very own nucleic acids and ribosomes. 4 chapters are dedicated to the nucleic acids and the ribosomal parts of either periods of those semi-independent lodgers. eventually, facts from a number of resources on genetic versions of enzymes are tabulated for prepared reference, and an overview of this data is attempted.

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Jones, 1973 Localisation of cellular globin messenger RNA by in situ hybridisation to complementary DNA. FEBS Lett. 32: 109-112. Harrison, P. , D. Conkie, N. Affara, and J. Paul, 1974 In situ localization of globin messenger RNA formation. I. During mouse fetal liver development. J. Cell Bioi. 63:402-413. Hearst, J. , T. R. Cech, K. A. Marx, A. Rosenfeld, and J. R. Allen, 1974 Characterization of the rapidly renaturing sequences in the main CsCI density bands of Drosophila, mouse, and human DNA.

Nail. Acad. Sci. USA 45:482-499. Firtel, R. A. and J. Bonner, 1972 Characterization of the genome of the cellular slime mold Dictyostelium discoideum. J. Mol. Biol. 66:339-361. Flamm, W. , 1972 Highly repetitive sequences of DNA in chromosomes. Int. Rev. Cytol. 32: 1-51. , B. Bierweiler, and H. W. Sauer, 1974 Reassociation kinetics of nuclear DNA from Physarum polycephalum. Eur. J. Biochem. 44:407-410. Gage, L. , 1974 The Bombyx mori genome: Analysis by DNA reassociation kinetics. Chromosoma 45:27-42.

However, it appears that the larger genomes have greater amounts of repeated DNA and have families of repeated sequences whose repetition frequency is higher than the smaller genome amphibians. Davidson et al. (1973) (Table 3) have studied the genome of Xenopus laevis (African clawed toad) in more detail, including an investigation into the interspersion of repetitive sequences with nonrepetitive sequences. By 22 Q. The Molecular Organization of Chromosomes a.. ~ u ~ u. 0 10 100 1000 10,000 Cot (mol X sec/liter) Figure 11.

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