Genome and Chromatin: Organization, Evolution, Function: by Prof. Dr. Walter Nagl (auth.), Prof. Dr. Walter Nagl, Prof.

By Prof. Dr. Walter Nagl (auth.), Prof. Dr. Walter Nagl, Prof. Dr. Vera Hemleben, Prof. Dr. Friedrich Ehrendorfer (eds.)

At a around desk dialogue at the eukaryotic chromosome sponsor­ ed through the Deutsche Forschungsgemeinschaft in Diisseldorf, February 1978, the botanists one of the members felt that plant platforms have been under-represented. during this unsatisfactory state of affairs, Professor V. HEMLEBEN, Tiibingen, steered one other assembly to debate genuine difficulties and effects touching on botanical chromosome examine. Professor W. NAGL used to be keen to arrange a symposium on the collage of Kaiserslautern, and Professor F. EHRENDoRFER, Wien, contacted the Rpringer-Verlag, Vienna-New York, to discover the potential for publishing the result of this symposium within the type of a complement quantity to the magazine Plant Systematics and Evol7ttion. The convention happened on 13-15 October 1978 within the division of Biology of the college of Kaiserslautern and used to be attended by way of forty members from eleven universities among Hamburg and Vienna. Emphasis of this Chromosome Symposium used to be given to 3 elements, which don't allure significant curiosity at huge foreign congresses: 1. dialogue and Demonstration of technical info which can't be present in released papers (so-called tricks). 2. Orientation approximately genuine developments and leads to our figuring out of the association, evolution, and serve as of the plant genome on the point of the DNA (gene), the extent of chromatin, and the extent of the karyotype. three. Presentation of hypotheses and types that may be stimulating for additional examine. additionally, more youthful scholars must have the prospect to provide their effects and to debate them with more matured scientists.

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Extra resources for Genome and Chromatin: Organization, Evolution, Function: Symposium, Kaiserslautern, October 13–15, 1978

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Acad. Sci. ) 75, 4451--4454. SUMNER, A. , BUCKLAND, R. , 1976: Relative DNA contents of somatic nuclei of ox, sheep, and goat. - Chromosoma 57, 171-175. , 1976: Physiology of polyploid plants: water balance in autotetraploid and diploid tomato under low and high salinity. - Physiol. Plant 38, 257-261. , 1972: Phylogenie chromosomique de l'homme et des primates hominiens (Pan troglodytes, Gorilla gorilla et Pongo pygmaeus). Essai de reconstitution du caryotype de l'ancestre commun. Ann. Genet. 15, 225-240.

1976: Evolution of repeated DNA sequences by unequal crossover. - Science 191, 528-535. STANLEY, S. , 1975: A theory of evolution above the species level. Nat. Acad. Sci. ) 72, 646-650. STEBBINS, G. , 1950: Variation and Evolution in Plants. - New York: Columbia Univer~it\" Prp~~. 1958: Longevity, habitat and release of genetic variability in the higher plants. - Cold Spring Harbor Symp. Quant. BioI. 23, 365-377. 1966: Chromosome variation and evolution. - Science 152, 1463-1469. 1971: Chromosomal Evolution in Higher Plants.

DNA Interspersion Experiments. Radioactively labelled tracer DNA of various fragment lengths was renatured with a vast excess (1 :5,000) of short driver DNA (average size: about 400 NT). These experiments were carried out in siliconized microcaps (50 [11). Palindromic reassociation was allowed to occur in 20mM NaPB (Ecot-values < 10-5 ). Renaturation of repetitive sequences was performed under conditions, which permit only repetitive DNA to react (up to an Ecot of 100, using 400mM NaPB). The incubation temperature was chosen to be 25°C below the Tm-value of native DNA, as demanded by WETMUR & al.

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