By Jichun Tian, Zhiying DENG, Kunpu Zhang, Haixia Yu, Xiaoling Jiang, Chun Li
The e-book as a rule describes the QTL mappings and efficacy analyses which are linked to wheat productiveness, caliber, body structure and diverse pressure resistances and gives summaries of effects from stories carried out either at domestic and abroad. It offers related info and analyses, supporting readers to reach at a extra complete figuring out of the newest improvement during this box. The e-book offers a wealth of novel info, large variety of purposes and in-depth findings on crop genetics and molecular breeding, making it priceless not just for plant breeders but in addition for educational colleges, senior researchers and complicated graduate scholars who're fascinated about plant breeding and genetics. Dr. Jichun Tian is a professor on the division of Agronomy, Shandong Agricultural college, Tai’an, China.
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Additional resources for Genetic Analyses of Wheat and Molecular Marker-Assisted Breeding, Volume 1: Genetics Map and QTL Mapping
Plant Mol Biol. 2005;57:461–85. Jaccoud D, Peng K, Feinstein D, Kilian A. Diversity arrays: a solid state technology for sequence information independent genotyping. Nucleic Acids Res. 2001;29:25–31. Kearsey MJ, Farquhar AG. QTL analysis in plants; where are we now? Heredity. 1998;80:137–42. Li G, Quiros CF. Sequence-related ampliﬁed polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet. 2001;103:455–61.
3 AFLP Markers AFLP was ﬁrst developed by Zabeau and Vos (1993). It combines the advantages of both RFLP and RAPD markers. The digested genomic DNA fragments with the two types of restriction nucleases produce sticky ends which are ligated with the synthetic double-stranded DNA. A subset of the restriction fragments is then selected to be ampliﬁed. The ampliﬁed fragments are separated and visualized on denaturing polyacrylamide gels, either through autoradiography or fluorescence methodologies, or via automated capillary sequencing instruments.
One of the key factors for successful DH production is to culture the anthers containing embryogenic microspores at mid-uninucleate to late uninucleate stages. Speciﬁcally, F1 hybrid’s anthers are harvested, in the ﬁeld prior to heading, when the spike length reaches 2/3 of leaf sheath in the northern winter wheat region, and young panicle length is at 1 cm of the auricles or spikes and auricles are equal in length in the south of Huanghuai district, respectively. Importantly, anther length is about 2/3 of its total length and anther should look green and opaque.