By Bernard L. Trumpower
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3A and 3B, and clearly shows that most of the cx is reduced prior to b reduction. Ob viously, this is because the Em of cx is higher than that of b. This figure also shows that the fumarate to succinate ratio affects the rates of reduc tion of the two cytochromes. An important observation, however, is that the Q-radical did not appear until more than half of the cx was reduced. This effect is very similar to what has been observed in the demonstration of the QP-C-radical(s) in the b-c-III complex (34,50) although radical ap pearance occurs before the reduction of b cytochromes.
However, it must be considered that both the hydroquinone and the water present in the solvent solution would have smaller disso ciation constants in the presence of 80% ethanol. The observed shift in the absorbance maximum of 18 nm from neu tral ubihydroquinone-10 to ubihydroquinone anion is within the range reported for other hydroquinones as is the ratio of the peak extinction coefficients (34) (Table I). Considering the average values for the red shifts and extinction-coefficient increases reported in forming the dianion hydroquinone from the anion for a variety of hydroquinones, pre dictions may be made concerning the spectrum of ubihydroquinone-10 dianion (34).
Morton, R. , Paterson, J. Y. , and Pennock, J. F. (1958). Biochem. J. 70, IP. Pumphrey, A. , Redfearn, E. , and Morton, R. A. (1958). Biochem. J. 70, IP. Green, D. E. (1959). Discuss. Faraday Soc. 27, 206-216. King, T. , and Chiang, Y. L. (1975). In "Electron Transfer Chains 24 Τ. Ε. King and Oxidative Phosphorylation" (E. Quagliariello, S. Papa, F. Palmieri, E. C. Slater, and N. ), pp. 105-118. , Amsterdam. 27. , and King, T. E. (1964). J. Biol. Chem. 239, 3546-3558. 28. King, T. , and Takemori, S.