By R. Fani, M. Bazzicalupo, E. Gallori, L. Turbanti, M. Polsinelli (auth.), Prof. Dr. Walter Klingmüller (eds.)
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Extra resources for Azospirillum III: Genetics · Physiology · Ecology Proceedings of the Third Bayreuth Azospirillum Workshop
Genet. in press 8. Franche, C. and Elmerich, C. (1981). Ann. Microbiol. (Inst. Pasteur) 132A, 3-19 9. Gauthier,D. and Elmerich, C. (1977). FEMS Microbiol. Lett. ~, 101-104 10. Pedrosa, F. G. (1984). FEMS Microbiol. Lett. 23, 95-101 11. Maniatis T. , Sambrook J. (1982) Molecular eloning, Cold Spring Harbor Laboratory 12. C. and Doly, J. (1979). Nuc. Acids Res. 2, 1513-1523 13. , Bigault, R. and Elmerich, C. (1980). Biochimie, 62, 267-275 14. , Rolfe, B. and Shine, J. (1981). J. Molee. AppI. Genet.
The Abr nif genes on pMS175-2 were then mutagenized with Tn1725 directly in MSO-8, a derivative of the mobilizing strain S17-1 (details to be published elsewhere). 0 kb away from the left and the right end of the Abr nif fragmente The exact locations of Tn1725 insertions were mapped (Fig. 2) as described in the previous section. The pMS175-2::Tn1725 derivatives were transferred to A. brasilense wild type strain 29145 rif. The insertion pMS1752::Tn 1725 No. 8 (in nifH) yielded nif- transconjugants.
Krishnapillai, V. and Merrick, M. (1977). Mol. Gen. Genet. 157, 189-198 40 15. Riedel, G. , AusubeI, F. M. and Cannon, F. C. (1979). Proc. Natl. Acad. Sci. USA 76, 2866-2870 16. Cannon, F. , Dixon, R. , Postgate, J. R. B. (1974). Mo!. Gen. Genet. 174, 59-66 17. Marmur, J. (1961). J. Mo!. Bio!. ~, 208-218 18. Rodriguez, R. L. and Tait, R. C. (1983). Recombinant DNA techniques. An introduction. Addison-Wesley publishing company, London 19. Singh, M. and KlingmOller, W. In: Trends in molecular gen~ tics.