Automated Enzyme Assays by D.B. Roodyn (Eds.)

By D.B. Roodyn (Eds.)

Within the face of speedy advancements in automatic options, this publication provides a good advisor to offer tendencies. It presents info on: rules and terminology of enzyme automation; computerized equipment illustrated through the Technicon approach; semi-automatic tools; interrupted-flow and discrete-sampling structures; single-enzyme research; multiple-enzyme research (M.E.A.); enzyme characterization; calculation of enzyme actions from device readings; generalized structures for enzyme automation; Appendices I. released computerized enzyme assays, II. Terminology utilized in enzyme automation, III. gear utilized in enzyme automation, IV. computing device application for generalized enzyme computerized method.

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An automatic digestor for the dermination of total nitrogen or phosphorus) and the reader is referred to the many brochures of the company for full details. Because of its widespread use, I will try to keep to the terminology used by Technicon and will use the term ‘flow system’ to describe the overall arrangement of the modules. 2. Automation of a simple-enzyme assay In the remaining chapters, I will often illustrate the various systems by examples from work from my laboratory. Many of the results were obtained in preliminary experiments during the development of the various systems and their scientific interest is of little relevance to this book.

Paper tape), either in addition to, or instead of, the pen recorder. 1. 6. Didlyzer This dialyzes the stream from the manifold against an appropriate medium and is used to separate low-molecular weight substances (such as phosphate) from protein for subsequent assay. The efficiency of concurrent dialysis is generally not very high, so that it cannot be used to separate all the low-molecular weight materials from the protein. (Much more efficient dialysis can be achieved by ‘counter current’ dialysis in which the donor and acceptor streams flow in opposite directions.

At the moment such systems are not widely used. g. various electrodes) most useful analytical methods would result. For example, simultaneous monitoring of oxygen concentration and levels of inorganic phosphate in mitochondria1 suspensions would provide a continuous measure of P/O ratios. Thus the combined use of multi-xensor reaction vessels with chemical analyzers could greatly increase the productivity of semi-automatic methods. 1. The Technicon autoanalyzer In compiling appendix I, the author has found that, a t the moment, the majority of published methods for enzyme automation are based on the Technicon system.

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